Table 2.

List of genes/constructs cloned into pEhAct-Neo

Plasmid	Encoded gene/construct
pOV-LGL1	Native lgl1 ORF.
pCYS-MUT	Mutated lgl1 ORF: Codons encoding C58, C61 and C62 were exchanged for codons encoding S58, T61 and S62.
pC-TRUNC	3′-truncated lgl1 ORF: Codons encoding amino acids 151–267 were removed. Therefore C228, C261, and the GPI addition site are absent.
pN-TRUNC	5′-truncated lgl1 ORF: Codons encoding amino acids 1–55 were removed. Codons for signal peptide (from −13 to −1) were preserved.
pDEEP-N	5′-truncated lgl1 ORF: Codons encoding amino acids 1–62 were removed. Therefore codons for C58, C61, and C62 are absent. Codons for signal peptide (from −13 to −1) were preserved.
pCONTROL	Mutated lgl1 ORF: an early stop codon was introduced.