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Expression and localization of myc-tagged SCAMP2 in Tet-regulated PC12 cells. Dox was added at 48 h after transfection, and cells were double-labeled with mouse anti-myc antibody and anti-secretogranin. Deconvolved images were prepared from five cells of each type expressing wild-type SCAMP2, mutant A (C→A), or mutant B (W→A). Representative optical sections (0.2 μm) including profiles of nuclei are shown. Bar, 10 μm.
