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. 2002 Dec;13(12):4279–4295. doi: 10.1091/mbc.E02-02-0105

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Copyright © 2002, The American Society for Cell Biology

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PLA cells induced toward the chondrogenic lineage synthesize a cartilagenous matrix and express genes consistent with the chondrogenic lineage. (A) PLA cells were induced in CM under high-density conditions for 14 d. Nodules were sectioned and stained with AB, in addition to antibodies to CNII, keratan sulfate (KS), and chondroitin-4-sulfate (CS). (B) PLA cells were induced for up to 3 wk in CM (PLA-CM). Sulfated proteoglycan levels were determined and normalized with respect to protein per microgram (PG). Non-induced PLA cells (PLA-control) were analyzed as a negative control. Values were expressed as the mean ± SD. (C) PLA nodules were induced in CM for up to 14 d or maintained in non-inductive control medium for 10 d (PLA-Con). Samples were analyzed by RT-PCR for the indicated genes. NHCK cells induced for 2 wk in CM were analyzed as a positive control. Dec, decorin.