Figure 7.

K82, which is important for interaction of Rab34 with RILP, is also essential for Rab34 to effect lysosomal positioning. (A) Alignment of amino acid sequences of mouse Rab34 (mRab34) and other human Rabs as indicated. The characteristic regions of small GTPases and K82 conserved among Rab34, Rab36, and Rab7 but not Rab6A′ or Rab33b are indicated. Residues highly conserved in all Rabs are indicated in red under yellow background, residues conserved in five of these six Rabs are indicated in blue under pink background, and those conserved in four Rabs are shown in brown under light blue background. (B) NRK cells transfected with EGFP-Rab34K82Q (a–c) and EGFP-Rab34F132Y (d–f) were processed for indirect immunofluorescence microscopy using anti-Lamp1 antibody. As shown, although both forms of Rab34 were enriched primarily in the Golgi apparatus, lysosomal redistribution was observed in cells expressing EGFP-Rab34F132Y but not EGFP-Rab34K82Q. Bar, 10 μm.