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RNAi against Ran and its cofactors affects targeting of nuclear pore proteins. Embryos from GFP::histone H2B worms grown on either control bacteria (A), or bacteria expressing dsRNA corresponding to Ran (B), RanGAP (C), RanBP2 (D), RCC1 (E), ima-2 (F), or imb-1 (G) were fixed and analyzed with the anti-nucleoporin mAb mAb414. Top row shows GFP::histone H2B in green, middle row shows mAb414 staining in red, and bottom row shows an overlay of the two signals. Bar, 10 μm.
