Table 1.
RNAi effects on brood size and viability
| C. elegans genea | Sequence | Human gene | PNb | nc | Brood sized | Lethality (%)e | |
|---|---|---|---|---|---|---|---|
| Controlf | 18 | 132 ± 37 | 0 ± 0 | ||||
| Ran | ran-1 | K01G5.4 | Ran | 1.6e−102 | 12 | 14 ± 16 | 100 ± 0 |
| RanGAP | ran-2 | C29E4.3 | RanGAP | 4.7e−73 | 7 | 140 ± 29 | 99 ± 1 |
| RanBP2 | npp-10 | F59A2.1 | RanBP2 | 2.6e−65 | 7 | 122 ± 60 | 100 ± 0 |
| RCC1 | ran-3 | C26D10.1 | RCC1 | 6.0e−34 | 10 | 151 ± 45 | 91 ± 13 |
| IMA-2 | ima-2 | F26B1.3 | Importin α | 5.3e−90 | 10 | 149 ± 43 | 99 ± 2 |
| IMA-3 | ima-3 | F32E10.4 | Importin α | 2.8e−116 | 9 | 35 ± 25 | 99 ± 2 |
| IMB-1 | imb-1 | F28B3.8 | Importin β | 9.8e−217 | 14 | 74 ± 60 | 100 ± 1 |
a
Left column shows gene product names used in this study; right column shows systematic gene name.
b
Probability values obtained in blast searches with human protein sequences against the WormPep database at http://www.wormbase.org.
c
Number of adult hermaphrodites analyzed for RNAi effects on brood size and embryonic lethality.
d
Average number of embryos being laid per hermaphrodite in 20 h ± SD. See MATERIALS AND METHODS for details.
e
Average percentage of embryos that do not hatch ± SD.
f
Control experiments were done by feeding the worms bacteria containing empty plasmid.