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Schematic representation of GFP-Rb fusion constructs (a). Transient expression of GFP-Rb fusion constructs in HEK293 cells. GFP-Rb fusion proteins were transiently expressed in HEK293 cells for 72 h. Cells were solublized in sample buffer and proteins analyzed by SDS-PAGE and immunoblotting by using monoclonal antibodies to GFP (b).
