Figure 2.

Cellular localization of GFP-Rb chimeras. HEK293 were transiently transfected with GFP-Rb fusion constructs and fixed with 4% formaldehyde with or without prior extraction with hypotonic buffer. Cells were processed for immunofluorescence microscopy. The DNA was stained with DAPI. Each micrograph shows the distribution of DNA (left) or GFP (middle) in black and white or merged images (right) in color. Rb-wt, wild-type Rb fused to GFP; N, N-terminal domain fused to GFP; NAB, N terminus + pockets A and B fused to GFP; C, pocket C fused to GFP. (a) Distribution of GFP chimeras in fixed cells. (b) Distribution of GFP chimeras in cells fixed after extraction with hypotonic buffers. (c) Distribution of endogenous Rb in cells fixed after hypotonic extraction. Bar, 10 μm.