. 2002 Dec;13(12):4401–4413. doi: 10.1091/mbc.E02-07-0450

Table 2.

Cellular distribution of GFP-Rb fusion constructs

Construct	Distribution before extraction		Distribution after extraction
Construct	Nuclear	Cytoplasmic	Nuclear	Cytoplasmic
GFP-wt	⁺	−	⁺	−
GFP-N	−	⁺	−	⁺
GFP-NA	−	⁺	−	⁺
GFP-NAB	⁺	⁺	−	⁺
GFP-A	−	⁺	−	⁺
GFP-AB	−	⁺	−	⁺
GFP-ABC	⁺	−	⁺	−
GFP-BC	⁺	−	⁺	−
GFP-C	⁺	−	⁺	−

GFP-Rb deletion constructs were expressed in HEK293 cells and their distribution between the nucleus, and the cytoplasm was observed before and after extraction with hypotonic buffer. + indicates the presence of a fusion protein within a compartment; − indicates the absence of fusion protein within a compartment.