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Sterol composition in raft domains of ELO3 cells. Comparison of the free sterol composition of total membranes (total) and the detergent-insoluble membrane fraction (rafts) in cells that have C26 substituted sphingolipids, but a mixture of different sterols (ELO3 erg6Δ p[ERG6]; YRS1551). Detergent-resistant membrane domains were isolated by two successive rounds of detergent extraction followed by flotation in density gradients. Sterol composition was analyzed by GC-MS.
