Figure 8.

elo3Δ affects the stability of newly synthesized Pma1p. (A) The half-life of newly synthesized Pma1p in wild-type (FY1679a), elo3Δ (YRS1118), and erg6Δ (YRS1266), and the elo3Δ erg6Δ double mutant strain bearing a plasmid-borne copy of either ERG6 (elo3Δ ERG6, YRS1520) or erg6^ts (elo3Δ erg6^ts, YRS1519) was assessed by pulse-chase analysis and immunoprecipitation. Cells were cultivated at 24°C or preshifted to 37°C for 15 min before labeling under the conditions indicated. (B) Pma1p degradation in the elo3Δ mutant requires PEP4 and END4. Turnover of newly synthesized Pma1p in elo3Δ pep4Δ (YRS1550) and elo3Δ end4Δ (YRS1554) double mutant strains was assessed as in A.