Table 5.
Total sterol composition of mutant strains at 24°C
| Wild-type | elo2Δ | elo3Δ | erg6Δ | elo3Δ ERG6 | elo3Δ erg6ts | |
|---|---|---|---|---|---|---|
| Zymosterol | 9.8 ± 1.3 | 14.6 ± 1.2 | 15.3 ± 1.8 | 35.4 ± 1.5 | 18.1 ± 2.5 | 45.4 ± 1.9 |
| Fecosterol | 9.3 ± 1.3 | 8.8 ± 0.4 | 14.1 ± 1.0 | n.d. | 14.4 ± 0.9 | n.d. |
| Cholesta-5,7,24-trienol | n.d. | n.d. | n.d. | 42.1 ± 2.5 | 10.1 ± 2.5 | 33.7 ± 2.6 |
| Cholesta-5,7,22,24-tetraenol | n.d. | n.d. | n.d. | 10.2 ± 0.1 | 2.0 ± 0.3 | 10.4 ± 0.6 |
| Ergosterol | 70.6 ± 1.4 | 63.4 ± 3.0 | 58.2 ± 2.9 | n.d. | 37.5 ± 2.3 | 1.3 ± 0.3 |
| Others | 8.7 ± 5.0 | 9.8 ± 3.4 | 7.9 ± 1.4 | 9.5 ± 1.0 | 12.2 ± 3.5 | 2.0 ± 0.6 |
n.d.; not detectable. Values are % of total. Other sterols are lanosterol, 14-desmethyl-lanosterol, 4-methyl-zymosterol, cholesta-5,8,24-trienol, episterol, cholesta-7,24-dienol, and ergosta-5,7-dienol.