Figure 9.

A region within L0 of Ycf1p, like MRP1, forms a helical wheel. (A) Alignment between MRP1 and Ycf1p indicating the complete L0 region, from amino acids 192 to 305 or 189 to 271, respectively, was performed using Clustal W software (Thompson et al., 1994). Black boxes represent amino acid identity and gray boxes indicate amino acid similarity as determined with the Boxshade server. The region proposed previously (Bakos et al., 2000) to form a helical wheel in MRP1 is indicated. (B) Computer predictions indicate that residues 223–235 of Ycf1p form an amphipathic helix (right). The predicted helical wheel for MRP1 (residues 221–233) is shown for comparison and represents published data (left; Bakos et al., 2000). The hydrophobic residues are shaded in gray.