Table 1.
Yeast strains used in this study
| Strain | Relevant genotype | Reference/source |
|---|---|---|
| BY4741 | MATa leu2Δ0 met15Δ0 ura3Δ0 his3Δ1 | (Brachmann et al., 1998) |
| MS10 | MATa ura3-52 leu2-3112 ade2-101 | M. Rose |
| MS1907 | MATa ura3-52 leu2-3112 ade2-101 pep4∷LEU2 | M. Rose |
| RH144-3D | MATa ura3 his4 leu2 bar1-1 | (Raths et al., 1993) |
| RH266-1D | MATa end3ts ura3 his4 leu2 bar1-1 | (Raths et al., 1993) |
| SM3851 | MATa trp1 leu2 ura3 his4 can1 ycf1Δ | (Sharma et al., 2002) |
| SM4516 | SM3851 [2μ URA3] | Transformant of SM3851 with pSM217 |
| SM4517 | SM3851 [2μ URA3 YCF1] | Transformant of SM3851 with pSM1752 |
| SM4518 | SM3851 [2μ URA3 YCF1-GFP] | Transformant of SM3851 with pSM1753 |
| SM4523 | MS10 [2μ URA3 YCF1-GFP] | Transformant of MS10 with pSM1753 |
| SM4524 | MS1907 [2μ URA3 YCF1-GFP] | Transformant of MS1907 with pSM1753 |
| SM4529 | RH144-3D [2μ URA3 YCF1-GFP] | Transformant of RH144-3D with pSM1753 |
| SM4531 | RH266-1D [2μ URA3 YCF1-GFP] | Transformant of RH266-1D with pSM1753 |
| SM4542 | SM3851 [2μ URA3 YCF1-HA-GFP] | Transformant of SM3851 with pSM1774 |
| SM4575a | MATa leu2Δ0 met15Δ0 ura3Δ0 his3Δ1 pep4∷KanMX4 | Research Genetics (Huntsville, AL) |
| SM4576a | MATa leu2Δ0 met15Δ0 ura3Δ0 his3Δ1 prb1∷KanMX4 | Research Genetics (Huntsville, AL) |
| SM4605 | BY4741 [2μ URA3 YCF1-GFP] | Transformant of BY4741 with pSM1753 |
| SM4606 | SM4575 [2μ URA3 YCF1-GFP] | Transformant of SM4575 with pSM1753 |
| SM4607 | SM4576 [2μ URA3 YCF1-GFP] | Transformant of SM4576 with pSM1753 |
| SM4735 | SM3851 [2μ URA3 ycf1Δ209-1515-HA] | Transformant of SM3851 with pSM1869 |
| SM4736 | SM3851 [2μ URA3 ycf1Δ272-1515-HA] | Transformant of SM3851 with pSM1870 |
| SM4741 | SM3851 [2μ URA3 ycf1Δ209-1515-HA] [2μ LEU2 ycf1Δ2-208-GFP] | Transformant of SM3851 with pSM1869, pSM1873 |
| SM4742 | SM3851 [2μ URA3 ycf1Δ272-1515-HA] [2μ LEU2 ycf1Δ2-271-GFP] | Transformant of SM3851 with pSM1870, pSM1874 |
| SM4758 | SM3851 [2μ URA3] [2μ LEU2] | Transformant of SM3851 with pSM217, pSM218 |
| SM4760 | SM3851 [2μ URA3 ycf1Δ209-1515-HA] [2μ LEU2] | Transformant of SM3851 with pSM1869, pSM218 |
| SM4761 | SM3851 [2μ URA3 ycf1Δ272-1515-HA] [2μ LEU2] | Transformant of SM3851 with pSM1870, pSM218 |
| SM4763 | SM3851 [2μ URA3] [2μ LEU2 ycf1Δ2-208-GFP] | Transformant of SM3851 with pSM217, pSM1873 |
| SM4764 | SM3851 [2μ URA3] [2μ LEU2 ycf1Δ2-271-GFP] | Transformant of SM3851 with pSM217, pSM1874 |
| SM4766 | SM3851 [2μ LEU2 ycf1Δ2-208-GFP] | Transformant of SM3851 with pSM1873 |
| SM4767 | SM3851 [2μ LEU2 ycf1Δ2-271-GFP] | Transformant of SM3851 with pSM1874 |
| SM4777 | SM3851 [2μ URA3] [2μ LEU2 YCF1-HA-GFP] | Transformant of SM3851 with pSM217, pSM1881 |
| SM4778 | SM3851 [2μ URA3 ycf1Δ209-1515-HA] [2μ LEU2 ycf1Δ2-271-GFP] | Transformant of SM3851 with pSM1869, pSM1874 |
| SM4876 | SM3851 [2μ URA3] [2μ LEU2 ycf1Δ223-235-HA-GFP] | Transformant of SM3851 with pSM217, pSM1890 |
a
This strain is a derivative of the strain BY4741 and was provided by J. Boeke (Johns Hopkins University School of Medicine, Baltimore, MD).