Figure 2.

MTs reorient toward the uropod after polarization by fMLF in suspended cells. Suspended PMNs in the absence of extracellular matrix coated substrate were stimulated with 10 nM fMLF for various times before fixation. The MT array (B, E, H, and K) and actin cytoskeleton (C, F, I, and L) were visualized by confocal microscopy. Differential interference contrast image (A, D, G, and J). Fluorescent images represent Z-axis confocal projections. Four minutes after fMLF stimulation (A–F) and 8 min after fMLF stimulation (G–L). Bar, 5 μm.