Figure 4.

Role of actin cytoskeleton in MT asymmetry. (A–F) Pattern of the MT array during “frustrated phagocytosis”. PMNs were plated on human nonimmune IgG for 4 min before fixation. (G–L) Pattern of the MT array after F-actin disruption. PMNs were plated on fibronectin (Fn)-coated coverslips, preincubated with 1 μM cytochalasin D for 4 min and stimulated with 10 nM fMLF plus cytochalasin D for 4 min before fixation. The MT array (B, E, H, and K) and actin cytoskeleton (C, F, I, and L) were visualized by confocal microscopy. Differential interference contrast image (A, D, G, and J). Fluorescent images represent Z-axis confocal projections. Bar, 5 μm.