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Actin expression in fibroblasts from BALF and bronchial biopsies from patients with SSc and mild asthma are arranged into stress fibers. Fibroblasts were cultured from bronchial biopsies and BALF from patients with SSc and mild asthma. Cells were seeded on four-well chamber slides (5000 cells/well), stained with Alexa Fluor™ 488 phalloidin showing stress fibers and analyzed using a fluorescence microscope.
