Figure 5.
Selective inhibition of ICAM-1 cross-linking induced RANTES mRNA in airway epithelial cultures by MAP kinase inhibitors (Figure A. A549 cells) (Figure B. NHBE cells). Cultures were pre-incubated in the presence of increasing concentrations of MAP kinase inhibitors and subsequently cross-linked for two hours with anti-ICAM-1 mAb and anti-mouse IgG F(ab')2 before RNA was isolated for RT-PCR analysis of RANTES message (40 PCR cycles). Messenger RNA of the 18s ribosomal gene was amplified under the same conditions and was used as the internal control. Lane 0 represents cultures that were cross-linked with control IgG1 mAb and anti-mouse IgG F(ab')2 for 2 hours. Cultures pre-incubated with MAP kinase inhibitors alone had no effect on RANTES or IL-8 mRNA. (n = 6; * = significantly different from control; † = significantly different from 0.5 μM PD98059; p < 0.05).