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. 2006 Feb 15;7:12. doi: 10.1186/1471-2202-7-12

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Copyright © 2006 Scholz et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Comparison of MHM transcripts. Alignment between the sequence of the EST clone RJA001B07 (787 nt) and the Z-linked MHM-region sequence AB046698 (2332 nt), showing a 141-nt region with 98% sequence similarity (blue boxes). The yellow fields are the regions of BLAT alignment to the Z-chromosome (RJA001B07: chrZ:9,422,551-9,422,655 and AB046698: chrZ:9,422,028-9,423,285) in the first assembly of the chicken genome [19]. Also indicated are the approximate locations of the two primer pairs used in the real-time PCR to confirm the higher expression in females of each MHM transcript.