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. Author manuscript; available in PMC: 2006 Mar 2.
Published in final edited form as: Biochemistry. 2005 Sep 27;44(38):12719–12727. doi: 10.1021/bi0510476

Figure 2.

Figure 2

(a) Postulated mechanism for the cyclization of farnesyl diphosphate (FPP) to trichodiene by trichodiene synthase; OPP = diphosphate, NPP = nerolidyl diphosphate (18). (b) R-azabisabolene, a cationic analogue of the bisabolyl cation in the trichodiene synthase mechanism, is a strong competitive inhibitor in the presence of inorganic diphosphate (PPi) with Ki = 0.51 μM (22); similarly, the enantiomer S-azabisabolene binds in the presence of PPi with Ki = 0.47 μM (22), suggesting that the stereochemical discrimination is weak at the corresponding step in catalysis.