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The effect of neutralizing positive charges of the RKKEE cluster on gating properties of MscL. A, B, and C show representative current traces and the corresponding pressure traces of MscL mutants where positive charges were neutralized (A and B) or protonated (C). (D) Boltzmann distribution fits calculated from the displayed traces showing rightward shift in the pressure axis in MscL mutants where positive charges were neutralized. See legend of Fig. 2 for details.
