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. 2005 Dec 16;90(6):2062–2074. doi: 10.1529/biophysj.105.071415

FIGURE 2.

FIGURE 2

The direct method yields proteoliposomes with more homogeneous size distributions. The diagrams show DLS analyses of the proteoliposomes used for the lipid mixing assays shown in Fig. 1 but diluted 100-fold (10 μM final lipid concentration). (A and E) Synaptobrevin proteoliposomes prepared by the standard method with a 160:1 lipid/protein ratio (A) or a 20:1 lipid/protein ratio (E). (B) Syntaxin/SNAP-25 proteoliposomes prepared by the standard method with a 150:1 lipid/protein ratio. (C and D) Synaptobrevin (C) and syntaxin/SNAP-25 (D) proteoliposomes prepared by the direct method with lipid/protein ratios of 185:1 and 200:1, respectively.