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Cross-linking study of arginine and aromatic substituted peptides in solution. Tricine 10–20% gradient polyacrylamide gel of cross-linking patterns NK4-M2GlyR-p22 derived peptides with aromatic and arginine amino acid substitutions (150 μM) treated with a 40-fold excess of cross-linking reagent in Ringers buffer “B”. Peptides treated with BS3 are labeled according to the sequence numbers given in Tables 2 and 3. The protein bands are visualized using silver stain.
