TABLE 1.
Characterization of M2GlyR-p22 derived peptides with C-terminal arginines
| Sequence | Replacement | Mr (Da) | Sol.(mM) | n | IscMAX (μA/cm2) | K1/2 (μM) |
|---|---|---|---|---|---|---|
| 1. KKKKPARVGLGITTVLTMTTQS | none | 2358.9 | 11.1 | 1.9 ± 0.6 | 23.7 ± 5.6 | 210 ± 70 |
| 2. KKKKPARVGLGITTVLTMTTQR | S22R | 2427.9 | 15.6 | 2.7 ± 0.9 | 24.1 ± 5.3 | 290 ± 60 |
| 3. KKKKPARVGLGITTVLTMTTRS | Q21R | 2387.0 | 19.7 | 2.2 ± 1.1 | 31.0 ± 18.0 | 390 ± 220 |
| 4. KKKKPARVGLGITTVLTMTRQS | T20R | 2414.0 | 18.7 | 1.3 ± 0.5 | 28.5 ± 14.8 | 310 ± 227 |
| 5. KKKKPARVGLGITTVLTMRTQS | T19R | 2414.0 | 18.3 | 0.7 ± 0.6 | 16.3 ± 3.5 | 120 ± 40 |
| 6. KKKKPARVGLGITTVLTRTTQS | M18R | 2383.9 | 23.3 | 0.6 ± 2.6 | 3.0 ± 4.0 | 840 ± 200 |
Sequences and physical and electrophysiological properties are shown. Bolded arginine residues define the putative TM segment. IscMAX is maximal anion flux and K1/2 is the concentration required to obtain one-half of the maximal anion flux calculated based on the Hill equation using the data presented in Fig. 2.