TABLE 3.
Characterization of peptides with phenylalanine/tyrosine and arginine substitutions
| Sequence | Replacement | Mr (Da) | Sol.(mM) | n | IscMAX (μA cm−2) | K1/2 (μM) |
|---|---|---|---|---|---|---|
| 12. KKKKPARVGLGITTVLTMTTRF | Q21R, S22F | 2447.1 | 6.1 | 3.8 ± 2.5 | 21.3 ± 2.5 | 56 ± 8 |
| 13. KKKKPARVGLGITTVLTMRTQF | T19R, S22F | 2474.1 | 12.2 | 2.3 ± 0.3 | 38.0 ± 2.3 | 120 ± 14 |
| 14. KKKKPARVGLGITTVLTMTTFR | Q21F, S22R | 2447.1 | 21.0 | 3.1 ± 0.6 | 31.8 ± 3.6 | 120 ± 10 |
| 15. KKKKPARVGLGITTVLTMTTRY | Q21R, S22Y | 2463.1 | 26.9 | 2.4 ± 0.6 | 29.4 ± 3.1 | 98 ± 14 |
| 16. KKKKPARVGLGITTVLTMRTQY | T19R, S22Y | 2490.1 | 24.5 | 2.3 ± 0.8 | 18.3 ± 2.9 | 110 ± 23 |
Sequences and physical and electrophysiological properties are shown. IscMAX is maximal anion flux and K1/2 is the concentration required to obtain one-half of the maximal anion flux and are calculated based on the Hill equation using the data presented in Fig. 6.