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. 2005 Dec 2;90(6):2235–2244. doi: 10.1529/biophysj.105.067165

FIGURE 5.

FIGURE 5

WT and mutant KCNQ1/KCNE1 (IKs) channel currents. (A) KCNQ1 was coexpressed with KCNE1 in Xenopus oocytes. IKs was elicited from a holding potential of −80 mV by 7-s pulses applied in 10-mV increments to potentials ranging from −120 to +70 mV. Tail currents were elicited by stepping to −120 mV. (B) I-V relationship for peak currents (n = 17–20). (C) Currents at +70 mV plotted versus side-chain volume of residue 336 (n = 17–20). (D) Fold-increase in KCNQ1 current magnitude (+70 mV) induced by coexpression with KCNE1 plotted versus side-chain volume of residue 336 (n = 11–20). (E) Normalized peak tail I-V relationship for IKs (n = 11–25). V1/2 values were A-336G/KCNE1 = 37.6 ± 1.9 mV, n = 11; A-336/KCNE1 = 48.3 ± 1.3 mV, n = 11; A-336C/KCNE1 = 43.1 ± 2.6 mV, n = 8; A-336T/KCNE1 = 41.1 ± 1.8 mV, n = 9.