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. 2002 Dec;1(6):1010–1020. doi: 10.1128/EC.1.6.1010-1020.2002

FIG. 1.

FIG. 1.

Analytical PCR of genomic DNA from C. albicans BWP17 parent strain and CET1 deletion strains. (Left panel) Schematic diagrams of wild-type or insertional deletion mutants for C. albicans chromosomal DNA at the CET1 locus with oligonucleotide binding sites and predicted PCR fragments. (Right panel) PCR fragments separated on 1% agarose gel. Lane M, DNA size standards; lane 1, C. albicans BWP17 genomic DNA PCR amplified with CaCET1F and CaCET1R primers; lane 2, C. albicans CQF141 genomic DNA PCR amplified with ARG4 and CaCET1R primers; lane 3, C. albicans CQF141 genomic DNA PCR amplified with CaCET1F and CaCET1R primers; lane 4, C. albicans CQF136 genomic DNA PCR amplified with ARG4 and CaCET1R primers; lane 5, C. albicans CQF136 genomic DNA PCR amplified with CaCET1F and CaCET1R primers; lane 6, C. albicans CQF160 genomic DNA PCR amplified with ARG4 and CaCET1R primers; lane 7, C. albicans CQF160 genomic DNA PCR amplified with CaCET1F and CaCET1R primers; lane 8, C. albicans CQF152 genomic DNA genomic DNA PCR amplified with CaCET1F and CaCET1R primers.