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. 2002 Dec;1(6):1010–1020. doi: 10.1128/EC.1.6.1010-1020.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 5. — TLC analysis shows altered mRNA cap structures for cet1- and ccm1-null mutants. TAP (A)- and P1 (B)-digested mRNA was purified from C. albicans BWP17, CQF152 (Δcet1::ARG4/Δcet1::URA), CQF248 (Δcet1::ARG4/Δcet1::URA3 with CET1 restored), CQF171 (Δccm1::ARG4/Δccm1::URA3) CQF250 (Δccm1::ARG4/Δccm1::URA3 with CCM1 restored), CQF142 (Δcet1::ARG4/CET1), andCQF154 (Δccm1::ARG4/CCM1), all labeled with NaB[³H]₄. The location of the standards separated on the TLC plate were visualized by UV light and are indicated on the right side of the image.