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Comitin is present in isolated phagosomal membranes. Lanes 1 through 3 show successive wash fractions of phagosomal preparations; the last lane represents protein of the phagosome fraction. Protein extracts were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (12% acrylamide), blotted onto nitrocellulose, and probed with the comitin-specific MAb 190-68-1. The 24-kDa signal of comitin decreased in the wash fractions and was present in the phagosome fraction.
