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Altered distribution of comitin in response to hyperosmotic shock. AX2 cells were incubated in 0.4 M sorbitol for the times indicated. The cells were allowed to settle on coverslips and fixed. Shown are cells after 90 min of incubation without (A) and with (B) sorbitol. The MAb 190-68-1 stained vesicles in the control cell, which were distributed throughout the cell. After treatment with sorbitol, the vesicles were enriched in the vicinity of the cell boundaries. Bar, 10 μm.
