FIG. 4.

Multinucleate B. dermatitidis yeast cells produce buds with one or more nuclei. Early-log-phase yeast cells of strain 26199 were harvested, fixed, RNase treated, and stained with Sytox Green. Panels are paired horizontally so that the same field is shown viewed with bright-field microscopy (A, C, E, G, and I) or fluorescence (B, D, F, H, and J, respectively). (A and B) Several multinucleate cells are shown. (C, D, E, and F) Budding pairs with septa and a single nucleus apparent in the bud. (G, H, I, and J) Budding pairs without septa and multiple nuclei in the bud. All panels are shown with the same magnification. A scale bar is shown in panel A.