FIG. 6.

Rvs161p-GFP localizes as cortical patches and concentrates at polarization sites. Wild-type cells grown in SD-casa medium were observed by Nomarski and fluorescence microscopy to visualize whole cells and Rvs161p-GFP localization, respectively. The equatorial view represents the middle of the cell. For the top view, the focusing was done on the top of the cell. (A) Unbudded cell, (B) bud emergence cell, and (C) budded cell with the septum. The arrow on the Nomarski image indicates the septum between the mother cell and the bud.