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. 2002 Dec;1(6):1021–1031. doi: 10.1128/EC.1.6.1021-1031.2002

FIG. 9.

FIG. 9.

Rvs161p is associated with DRMs. (A) Wild-type cells were treated as described in Materials and Methods. Rvs161p was immunoprecipitated from the membrane (M), the detergent-resistant membrane (R), and the respective soluble (S) fractions with specific antibodies, followed by SDS-PAGE and Phosphorimager analysis. (B) The lcb1-100 mutant and the corresponding wild-type (WT, RH1800) strain were grown at the permissive (24°C) or the restrictive (37°C) temperature and treated as described in Materials and Methods. Rvs161p was immunoprecipitated from detergent-resistant membrane (R) and soluble (S) fractions with specific antibodies and analyzed by SDS-PAGE and Phosphorimager analysis.