TABLE 1.
S. cevevisiae strains used in this studya
| Strain | Relevant genotype | Source or reference |
|---|---|---|
| BY4742 | α his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | EUROSCARF |
| Y13489 | α rvs161Δ leu2Δ0 lys2Δ0 ura3Δ0 his3Δ1 | EUROSCARF |
| LG973-1B | a rvs161Δ his3Δ1 leu2Δ0 ura3Δ0 | This study |
| LG742-17A | a act1-1 his4 | 5 |
| LG1041-6B | α rvs161Δ fen1Δ ura3Δ0 his3Δ1 leu2Δ0 | This study |
| LG1040-6A | a rvs161Δ sur4Δ ura3Δ0 his3Δ1 leu2Δ0 | This study |
| LG1043-6B | a rvs161Δ sur2Δ ura3Δ0 his3Δ1 leu2Δ0 | This study |
| LG1034-20C | a rvs161Δ sur1Δ ura3Δ0 his3Δ1 leu2Δ0 | This study |
| LG1035-6B | a rvs161Δ ipt1Δ ura3Δ0 his3Δ1 leu2Δ0 | This study |
| LG1039-2B | α act1-1 fen1Δ leu2Δ0 lys2Δ0 his-? | This study |
| LG1045-8C | a act1-1 sur4Δ leu2Δ0 ura3Δ0 | This study |
| LG1044-9A | a act1-1 sur2Δ his-? | This study |
| LG1036-10D | α act1-1 sur1Δ his leu2Δ0 lys2Δ0 ura3Δ0 | This study |
| LG1037-3C | α act1-1 ipt1Δ his leu2Δ0 | This study |
| RH1800 | α leu2 his4 ura3 bar1 | H. Riezman |
| RH3804 | α lcb1-100 trp1 leu2 ura3 lys2 | H. Riezman |
All strains are in the S288C background or are congenic except the lcb1-100 strain. All the EUROSCARF disruptant strains are Kanr and are derived from BY4742 and therefore bear the same auxotrophies. The disruptions, noted Δ, are kanMX4. The segregant LG973 is from Y13489 × LG811-8A (5). The fen1, sur4, sur2, sur1, and ipt1 mutants are strains from EUROSCARF. The double mutants LG1041-6B, LG1040-6A, LG1034-20C, LG1043-6B, and LG1035-6B come from a cross between LG973-1B and the EUROSCARF strains. Backcrosses were made to check for the segregation of the Kanr markers. The double mutants containing act1-1 are from a cross between LG742-17A and the EUROSCARF strains. The presence of the act1-1 allele was attested by complementation tests with plasmids containing the wild-type FEN1, SUR4, and SUR1 genes. The wsc1, wsc2, wsc3, wsc4, and mid2 disruptants are from EUROSCARF.