TABLE 1.
Genes that are upregulated by a factor of 2 or more in response to a disruption of the PEX12 genea
Gene | ORFb | Extent of upregulation (factor) | Function | PTS | Lysine-auxotroph? |
---|---|---|---|---|---|
LYS9 | YNR050C | 5.06 | Saccharopine dehydrogenase | -SLx5HP- | Yes |
LYS20 | YDL182W | 3.96 | Homocitrate synthase | Yes | |
CTP1 | YBR291C | 3.39 | Citrate transporter (mitochondrial) | Partially† | |
LYS4 | YDR234W | 2.99 | Homoaconitase | -SQL* | Yes |
SNO1 | YMR095C | 2.83 | Stationary-phase induced gene | No | |
YJL200C | YJL200C | 2.74 | Aconitase homologue | Partially† | |
LYS1 | YIR034C | 2.56 | Saccharopine dehydrogenase | -SRL* | Yes |
LYS12 | YIL094C | 2.47 | Homoisocitrate dehydrogenase | -SRL | Yes |
YBR147W | YBR147W | 2.45 | STM1-homologue, 7-TM nitrogen starvation sensor | No | |
SNZ1 | YMR096W | 2.26 | Stationary-phase induced gene | No | |
YDL183C | YDR183C | 2.07 | Uncharacterized | No | |
LYS21 | YDL131W | 1.64 | Homocitrate synthase | Yes | |
LYS2 | YBR115C | 1.57 | Aminoadipate semialdehyde dehydrogenase | -KLx5HL- | Yes |
LYS5 | YGL154C | 1.17 | Lys2p-phosphopantetheinyl transferase | Yes |
For reference, three other genes involved in lysine biosynthesis are shown at the bottom of the table. Functional properties according to the Saccharomyces Genome Database (http://genome-www.stanford.edu/Saccharomyces/). PTS, predicted peroxisomal targeting signal. Peroxisomal targeting has been shown by GFP fusion (14) and is indicated by an asterisk. The doubling time is increased by ca. 150% in YJL200C-disrupted and 10% in CTP1-disrupted yeast in lysine-deficient medium and is indicated by a dagger.
ORF, open reading frame.