FIG. 1.

Azole-dependent upregulation of ERG11. RNA slot blot hybridization was used to examine ERG11 expression in strain W303-1A following treatment with nocodazole (3 μg/ml), fluconazole (20 μg/ml), miconazole (0.3 μg/ml), or dimethyl sulfide (DMSO; drug vehicle) for the times indicated in the figure. ACT1 expression was employed as a loading control. Cells were cultured in YPD medium. Probes were prepared from PCR products generated with the primers indicated in Table 1.