FIG. 2.

ROX1 deletion results in increased expression of multiple ERG genes. RNA slot blot hybridization was used to measure the expression of the indicated ERG genes in strains YJN433 (solid bars) and YJN433 rox1Δ (stippled bars). RNA was extracted from mid-log-phase cultures (2 × 10⁷ cells/ml) (unshaded bars) or late-log-phase cultures (1 × 10⁸ cells/ml) (shaded bars). Autoradiographs were densitometrically scanned; values shown represent the fold change in RNA relative to the levels in mid-log YJN433 cultures, with normalization to ACT1 RNA levels.