FIG. 3.
ERG11 expression is inversely correlated with ROX1 expression. RT-PCR (odd-numbered lanes) was used to examine ROX1, ERG11, and ACT1 expression in untreated (lanes 3 to 11) or fluconazole-treated (9 μg/ml) (lanes 13 to 21) cultures of strain W303-1A. Log-phase cultures (3 × 107 cells per ml) were sampled after incubation for 0 h (lane 1), 0.25 h (lanes 3 and 13), 0.5 h (lanes 5 and 15), 1 h (lanes 7 and 17), 2 h (lanes 9 and 19), or 5 h (lanes 11 and 21). Control reactions which lacked RT (even-numbered lanes) confirmed that the observed bands were not due to genomic DNA contamination. Lane G is a positive control from a reaction mixture containing genomic DNA. Amplification was for 23 (ROX1 and ERG11) or 25 (ACT1) cycles, which was within the logarithmic range. Primers are indicated in Table 1.