TABLE 1.
Primers used in this studya
| Gene | GenBank accession no. | Position (bp) | Primer pair | Sequence (5′ to 3′) |
|---|---|---|---|---|
| ACT1 | L00026 | 1297-1811 | ACT2000F | ACCGAAGCTCCAATGAATCCAAAATCC |
| ACT2516R | GTTTGGTCAATACCAGCAGCTTCCAAA | |||
| ROX1 | X60458 | 545-1295 | ROX1F | CAATCAACAATGAATCCTAAATC |
| ROX1R | TTACCGGTGTTTGACTGCTG | |||
| ROX1, His5+ | X60458 | 503-1701 | ROX1ΔF | AGAAAATACTAATACTTCACACAAAAGAAACGCAGTAGACAATCAACGGATCCCCGGGTTAATTAA |
| ROX1ΔR | ATAATATATATAACGGAAAGAAGAAATGGAAAAAAAAAATCATTTCGGATGAATTCGAGCTCGTTTACAC | |||
| ERG11 | M18109 | 1762-2266 | ERG11F | ATTGGTATTCTTATGGGTGGTCAACATAC |
| ERG11R | CCCAATACATCTATGTCTACCACCACC | |||
| ERG1 | M64994 | 1085-1747 | ERG1F | TTGACAATTAGTTGTGATGGTAT |
| ERG1R | CTTTGGAAATATTTGAAACAACC | |||
| ERG3 | M62623 | 1275-1793 | ERG3F | CCWMTTTGAAAAACCAAATG |
| ERG3R | GAATTGACCGTAGTTGTAGTTGAA | |||
| ERG7 | U04841 | 2424-2988 | ERG7F | TATCCATACGTGGAATGTAC |
| ERG7R | TGTATAWACCTAATGCCTTAAT | |||
| ERG9 | X59959 | 778-1231 | ERG9F | AAAATGGGTAATGGTATGGC |
| ERG9R | CTTGYGGAATYGCACAAAAT |
a
For each gene, the GenBank accession number and the corresponding termini (base pair position) of the PCR product are indicated. Nucleotides that are complementary to the regions flanking the His5+ gene of plasmid pFA6a-His5MX6 are underlined. ROX1ΔF corresponds to the ROX1 sequence immediately upstream of the start codon; ROX1ΔR overlaps the stop codon.