TABLE 2.
Effect of ste mutations on FUS1 expression and morphology of the bud14 mutant
| Strain | Genotype | FUS1 expressiona | AT (mM)b | Cell morphology |
|---|---|---|---|---|
| SY2431 | ste4Δ | 0.1 | His− | WTd |
| SY3871 | ste4Δ bud14-1 | 0.5 | His+ | Elongated |
| SY3874 | ste4Δ bud14Δ | 0.45 | His+ | Elongated |
| SY3875 | ste5Δ bud14Δ | 0.5 | His+ | Elongated |
| SY3876 | ste20Δ bud14Δ | 0.25 | His− | Irregular |
| SY3877 | ste50Δ bud14Δ | 0.1 | His− | Elongated |
| SY3878 | ste11Δ bud14Δ | 0.1 | His− | Elongated |
| SY3879 | ste12Δ bud14Δ | 0.1 | His− | Elongated |
| SY2002 | Wild type | 19 | His+ | WT |
| SY3873 | bud14Δ | 33 | 1 | Elongated |
| SY3912 | rga1Δ | 48 | 1 | Elongated |
| SY3880 | bud14Δ rga1Δ | 193 | 15 | Irregular |
| SY2002 | Wild typec | 18 | His+ | WT |
| SY3913 | pbs2Δc | 50 | 1 | WT |
| SY3913 | bud14Δc | 38 | 1 | Elongated |
| SY3880 | bud14Δ pbs2Δc | 444 | 15 | Irregular |
a
β-Galactosidase activity was measured as described in Materials and Methods. The reported values are the average of three independent determinations, and standard deviation was less than 20%.
b
The highest concentration of AT (aminotriazole) tested that allowed growth on synthetic medium lacking histidine. His+ refers to growth on SD-His medium but not on SD-His plus 1 mM AT.
c
Cells were grown in synthetic medium, which showed the maximal induction of the bud14 pbs2 double mutant.
d
WT, wild-type morphology.