TABLE 3.
Bud-site-selection defect in the haploid bud14 mutanta
| Strain | % Cells with scar type
|
||
|---|---|---|---|
| Axial | Random | Bipolar | |
| Wild type | 87 | 4 | 9 |
| bud14 | 27 | 13 | 60 |
a
Wild-type (SY2002) and bud14 (SY3873) cells were grown to mid-log phase in YPD at 30°C and stained with Calcofluor as described in Materials and Methods. Cells containing multiple bud scars were classified as axial (three or more scars at one pole), random (at least one scar in the middle third of the cell), or bipolar (three or more scars at both poles); at least 200 cells were counted for each strain.