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RNA blot analysis of mfa1 transcript levels in ras2 mutants. Total RNA was isolated from 002 Δras2 cells, wild-type 002 cells carrying the control vector pHyg101, and wild-type 002 cells carrying the activated ras2Val16 allele in prV16Hyg. The RNA blot was hybridized with a probe for the mfa1 gene and exposed for 3.75 h (A) or stained with 0.04% methylene blue (B).
