FIG. 9.

Cellular phenotype of mutants with defects in Ras2 and cAMP or MAP kinase signaling. Wild-type 518 and ubc1, hgl1, and ubc3 mutant cells were transformed with the vector control pHyg101 (left column) or a plasmid containing the activated ras2^Val16 allele, prV16Hyg (right column). fuz7 and prf1 mutant cells were transformed with a vector control, pSat112 (left column), or a plasmid containing the activated ras2^Val16 allele, prV16Sat (right column), conferring resistance to the antibiotic nourseothricin.