Skip to main content
PMC home page
British Journal of Clinical Pharmacology logoLink to British Journal of Clinical Pharmacology
. 1987 Aug;24(2):139–144. doi: 10.1111/j.1365-2125.1987.tb03154.x

The actions of calcitonin gene related peptide and vasoactive intestinal peptide as vasodilators in man in vivo and in vitro.

S M Thom, A D Hughes, P Goldberg, G Martin, M Schachter, P S Sever
PMCID: PMC1387742  PMID: 3497661

Abstract

1 The two peptides calcitonin gene related peptide (CGRP) and vasoactive intestinal peptide (VIP) produced marked dilatation of the forearm vascular bed when infused via the brachial artery. 2 CGRP relaxed preconstricted segments of human radial, coronary, gastric and cerebral arteries in an endothelium dependent manner. 3 VIP relaxed human gastric and transverse cervical arteries in an endothelium dependent manner, but relaxation of the human pulmonary artery was not dependent on endothelium. 4 The characteristics of the endothelium dependent relaxation of these medium-sized muscular arteries indicated involvement of the endothelium derived relaxing factor in vitro. 5 Caution is expressed in drawing comparisons between the mechanisms involved in the in vivo and in vitro vascular responses.

Full text

PDF
142

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amara S. G., Jonas V., Rosenfeld M. G., Ong E. S., Evans R. M. Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products. Nature. 1982 Jul 15;298(5871):240–244. doi: 10.1038/298240a0. [DOI] [PubMed] [Google Scholar]
  2. Brain S. D., Williams T. J., Tippins J. R., Morris H. R., MacIntyre I. Calcitonin gene-related peptide is a potent vasodilator. Nature. 1985 Jan 3;313(5997):54–56. doi: 10.1038/313054a0. [DOI] [PubMed] [Google Scholar]
  3. Burnstock G. Nervous control of smooth muscle by transmitters, cotransmitters and modulators. Experientia. 1985 Jul 15;41(7):869–874. doi: 10.1007/BF01970003. [DOI] [PubMed] [Google Scholar]
  4. D'Orléans-Juste P., Dion S., Mizrahi J., Regoli D. Effects of peptides and non-peptides on isolated arterial smooth muscles: role of endothelium. Eur J Pharmacol. 1985 Aug 7;114(1):9–21. doi: 10.1016/0014-2999(85)90515-1. [DOI] [PubMed] [Google Scholar]
  5. Fisher L. A., Kikkawa D. O., Rivier J. E., Amara S. G., Evans R. M., Rosenfeld M. G., Vale W. W., Brown M. R. Stimulation of noradrenergic sympathetic outflow by calcitonin gene-related peptide. Nature. 1983 Oct 6;305(5934):534–536. doi: 10.1038/305534a0. [DOI] [PubMed] [Google Scholar]
  6. Galbo H., Hilsted J., Fahrenkrug J., Schaffalitzky De Muckadell O. B. Fasting and prolonged exercise increase vasoactive intestinal polypeptide (VIP) in plasma. Acta Physiol Scand. 1979 Mar;105(3):374–377. doi: 10.1111/j.1748-1716.1979.tb06353.x. [DOI] [PubMed] [Google Scholar]
  7. Girgis S. I., Macdonald D. W., Stevenson J. C., Bevis P. J., Lynch C., Wimalawansa S. J., Self C. H., Morris H. R., MacIntyre I. Calcitonin gene-related peptide: potent vasodilator and major product of calcitonin gene. Lancet. 1985 Jul 6;2(8445):14–16. doi: 10.1016/s0140-6736(85)90059-5. [DOI] [PubMed] [Google Scholar]
  8. Goodman E. C., Iversen L. L. Calcitonin gene-related peptide: novel neuropeptide. Life Sci. 1986 Jun 16;38(24):2169–2178. doi: 10.1016/0024-3205(86)90568-0. [DOI] [PubMed] [Google Scholar]
  9. Guild S., Drummond A. H. Vasoactive-intestinal-polypeptide-stimulated adenosine 3',5'-cyclic monophosphate accumulation in GH3 pituitary tumour cells. Reversal of desensitization by forskolin. Biochem J. 1984 Aug 1;221(3):789–796. doi: 10.1042/bj2210789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kubota M., Moseley J. M., Butera L., Dusting G. J., MacDonald P. S., Martin T. J. Calcitonin gene-related peptide stimulates cyclic AMP formation in rat aortic smooth muscle cells. Biochem Biophys Res Commun. 1985 Oct 15;132(1):88–94. doi: 10.1016/0006-291x(85)90992-1. [DOI] [PubMed] [Google Scholar]
  11. Lundberg J. M., Franco-Cereceda A., Hua X., Hökfelt T., Fischer J. A. Co-existence of substance P and calcitonin gene-related peptide-like immunoreactivities in sensory nerves in relation to cardiovascular and bronchoconstrictor effects of capsaicin. Eur J Pharmacol. 1985 Feb 5;108(3):315–319. doi: 10.1016/0014-2999(85)90456-x. [DOI] [PubMed] [Google Scholar]
  12. Martin W., Villani G. M., Jothianandan D., Furchgott R. F. Selective blockade of endothelium-dependent and glyceryl trinitrate-induced relaxation by hemoglobin and by methylene blue in the rabbit aorta. J Pharmacol Exp Ther. 1985 Mar;232(3):708–716. [PubMed] [Google Scholar]
  13. Mulderry P. K., Ghatei M. A., Rodrigo J., Allen J. M., Rosenfeld M. G., Polak J. M., Bloom S. R. Calcitonin gene-related peptide in cardiovascular tissues of the rat. Neuroscience. 1985 Mar;14(3):947–954. doi: 10.1016/0306-4522(85)90156-3. [DOI] [PubMed] [Google Scholar]
  14. Parnavelas J. G., Kelly W., Burnstock G. Ultrastructural localization of choline acetyltransferase in vascular endothelial cells in rat brain. Nature. 1985 Aug 22;316(6030):724–725. doi: 10.1038/316724a0. [DOI] [PubMed] [Google Scholar]
  15. Rapoport R. M., Draznin M. B., Murad F. Endothelium-dependent relaxation in rat aorta may be mediated through cyclic GMP-dependent protein phosphorylation. Nature. 1983 Nov 10;306(5939):174–176. doi: 10.1038/306174a0. [DOI] [PubMed] [Google Scholar]
  16. Rosenfeld M. G., Mermod J. J., Amara S. G., Swanson L. W., Sawchenko P. E., Rivier J., Vale W. W., Evans R. M. Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processing. Nature. 1983 Jul 14;304(5922):129–135. doi: 10.1038/304129a0. [DOI] [PubMed] [Google Scholar]
  17. Said S. I., Mutt V. Polypeptide with broad biological activity: isolation from small intestine. Science. 1970 Sep 18;169(3951):1217–1218. doi: 10.1126/science.169.3951.1217. [DOI] [PubMed] [Google Scholar]
  18. Said S. I., Mutt V. Potent peripheral and splanchnic vasodilator peptide from normal gut. Nature. 1970 Feb 28;225(5235):863–864. doi: 10.1038/225863a0. [DOI] [PubMed] [Google Scholar]
  19. Stevenson J. C., Macdonald D. W., Warren R. C., Booker M. W., Whitehead M. I. Increased concentration of circulating calcitonin gene related peptide during normal human pregnancy. Br Med J (Clin Res Ed) 1986 Nov 22;293(6558):1329–1330. doi: 10.1136/bmj.293.6558.1329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Struthers A. D., Brown M. J., Macdonald D. W., Beacham J. L., Stevenson J. C., Morris H. R., MacIntyre I. Human calcitonin gene related peptide: a potent endogenous vasodilator in man. Clin Sci (Lond) 1986 Apr;70(4):389–393. doi: 10.1042/cs0700389. [DOI] [PubMed] [Google Scholar]
  21. Tschopp F. A., Henke H., Petermann J. B., Tobler P. H., Janzer R., Hökfelt T., Lundberg J. M., Cuello C., Fischer J. A. Calcitonin gene-related peptide and its binding sites in the human central nervous system and pituitary. Proc Natl Acad Sci U S A. 1985 Jan;82(1):248–252. doi: 10.1073/pnas.82.1.248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Uddman R., Alumets J., Edvinsson L., Håkanson R., Sundler F. VIP nerve fibres around peripheral blood vessels. Acta Physiol Scand. 1981 May;112(1):65–70. doi: 10.1111/j.1748-1716.1981.tb06783.x. [DOI] [PubMed] [Google Scholar]
  23. Vegesna R. V., Diamond J. Effects of isoproterenol and forskolin on tension, cyclic AMP levels, and cyclic AMP dependent protein kinase activity in bovine coronary artery. Can J Physiol Pharmacol. 1984 Sep;62(9):1116–1123. doi: 10.1139/y84-187. [DOI] [PubMed] [Google Scholar]
  24. WHITNEY R. J. The measurement of volume changes in human limbs. J Physiol. 1953 Jul;121(1):1–27. doi: 10.1113/jphysiol.1953.sp004926. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wei E. P., Kontos H. A., Said S. I. Mechanism of action of vasoactive intestinal polypeptide on cerebral arterioles. Am J Physiol. 1980 Dec;239(6):H765–H768. doi: 10.1152/ajpheart.1980.239.6.H765. [DOI] [PubMed] [Google Scholar]
  26. Weihe E., Reinecke M. Peptidergic innervation of the mammalian sinus nodes: vasoactive intestinal polypeptide, neurotensin, substance P. Neurosci Lett. 1981 Nov 4;26(3):283–288. doi: 10.1016/0304-3940(81)90146-4. [DOI] [PubMed] [Google Scholar]
  27. Wohlwend A., Malmström K., Henke H., Murer H., Vassalli J. D., Fischer J. A. Calcitonin and calcitonin gene-related peptide interact with the same receptor in cultured LLC-PK1 kidney cells. Biochem Biophys Res Commun. 1985 Sep 16;131(2):537–542. doi: 10.1016/0006-291x(85)91269-0. [DOI] [PubMed] [Google Scholar]
  28. Zaidi M., Bevis P. J., Girgis S. I., Lynch C., Stevenson J. C., MacIntyre I. Circulating CGRP comes from the perivascular nerves. Eur J Pharmacol. 1985 Nov 5;117(2):283–284. doi: 10.1016/0014-2999(85)90616-8. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Clinical Pharmacology are provided here courtesy of British Pharmacological Society

RESOURCES