Table 3.

Influence of SYBR Green I concentration on the melting temperature of the PCR product specific for F. graminearum. 50 pg of Fg3 DNA were used as a template for PCR with the primer pair Fg16N F/R in addition to different SYBR Green I concentrations and 3 mM MgCl₂. The other parameters were set as described in the Methods.

SYBR Green I concentration in the amplification mix	Melting temperature of the 280 bp PCR product
0.1×	84.5°C
0.2×	85.0°C
0.3×	85.5°C
0.4×	86.0°C
0.7×	86.5°C