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. Author manuscript; available in PMC: 2006 Mar 3.
Published in final edited form as: Circ Res. 2006 Jan 26;98(4):472–479. doi: 10.1161/01.RES.0000204725.46332.97

Figure 4.

Figure 4

CIITA downregulates collagen type I promoters and activates MHC II promoter in human SMCs. A, COL1A1 promoter construct (−311/+114) (0.5 μg) was cotransfected with GFP construct (0.1 μg) and/or pcDNA3 empty vector or CIITA type I, III, and IV expression vectors (0.5 μg) into human aortic SMCs. B, COL1A2 promoter construct (pGL3-Col-Luc, −357/+55) (0.5 μg) was cotransfected with GFP construct (0.1 μg) and/or pcDNA3 empty vector or CIITA type I, III, and IV expression vectors as in A. C, pGL3-HLA-DRA promoter construct (pGL-300-DRα) was cotransfected with GFP construct and either pcDNA3 empty vector or CIITA type I, III, and IV expression vectors as in A. Luciferase activity, corrected for transfection efficiency with GFP, was normalized by protein concentration and is shown as the mean±SD (n=3). The data were evaluated for significance by ANOVA followed by Sheffe's post hoc analysis using SPSS software (*P<0.01).