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. 2006 Mar 2;34(4):1281–1292. doi: 10.1093/nar/gkl016

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© The Author 2006. Published by Oxford University Press. All rights reserved

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STM/BLH interactions in yeast and co-immunoprecipitations. (A) Galactosidase activity mediated by the interaction of the MEINOX domain cloned into pGBKT7 (bait) with BLH partner proteins expressed in pACT2. No signal is observed with the empty bait vector. The positive controls upper panel to the left shows the interaction between p53 and the SV40 T-antigen (pGBKT7-53 and pADT7-T, respectively, provided in the MATCHMAKER Biosensor kit). (B) In vitro translated full-length BLH proteins tagged with the hemaglutine (HA) epitope which were used in co-precipitation experiments with His-tagged STM protein and visualized by an anti-HA HRP-conjugated antibody. (C) Co-precipitated STM-His protein detected by a penta-His antibody. Asterisks mark control lanes showing that STM-His is undetectable in immunoprecipitations in the absence of BLH partner proteins.