TABLE 5.
VPC isolation experiments
| Genotypea | VPC | Vulval fate | Nonvulval fate |
|---|---|---|---|
| lin-12(ΔE)∷gfp | P4.p (isolated) | 6 | 0 |
| lin-12(ΔE)∷gfp | P4.p (unoperated) | 7 | 0 |
| bre-5(ye17); lin-12(ΔE)∷gfp | P4.p (isolated) | 11 | 0 |
| bre-5(ye17); lin-12(ΔE)∷gfp | P4.p (mock operated)b | 4 | 12 |
| lin-12(ΔE)∷gfp | P8.p (isolated) | 12 | 0 |
| lin-12(ΔE)∷gfp | P8.p (unoperated) | 7 | 0 |
| bre-5(ye17); lin-12(ΔE)∷gfp | P8.p (isolated) | 9 | 0 |
| bre-5(ye17); lin-12(ΔE)∷gfp | P8.p (mock operated)b | 6 | 10 |
a
These animals are also lin-12(+). Each unoperated animal of either genotype has a normal anchor cell and forms a functional vulva.
b
Mock-operated animals were treated in exactly the same way as laser operated ones, but no VPCs were ablated. Each VPC was scored as adopting either a vulval or a nonvulval fate; all pseudovulvae that were observed had the characteristic morphology associated with the 2° fate.