Table 2.

Copper subcellular distribution in the root cell of plants exposued to 0.25 and 500 µmol/L Cu (mg/kg FW)

Fractions	Cu subcellular localization
	0.25 µmol/L Cu (control)		500 µmol/L Cu
	8 d	16 d	8 d	16 d
Cell wall	7.82 (47)*	8.75 (45)	1038 (56)	2380 (61)
Plastid	4.17 (25)	5.69 (29)	265 (14)	558 (14)
Nucleus	0.91 (5.6)	1.05 (5.3)	90.3 (4.9)	162 (4.2)
Mitochondrion	0.96 (5.7)	1.02 (5.2)	84.7 (4.6)	126 (3.2)
Ribosome	1.12 (6.7)	1.27 (6.5)	83.2 (4.5)	103 (2.6)
Soluble fraction	1.85 (11)	1.91 (9.7)	295 (16)	573 (15)
Total Cu	19.6	23.5	2024	3804
Percentage recovery (%)#	86%	84%	92%	103%

^*Values in the bracket represent distribution percent (%)

^#Percentage recovery (%)=(Cell wall+Plastid+Nucleus+Mitochondrion+Ribosome+Soluble fraction)/Total