Table 1.
Specificity of the primary antibodies used, and corresponding subcellular localization and degree of staining observed for ovotestis
| Staining of ovotestis | ||||
| Male germ cells | ||||
| Antibodies | Specificities | Sources | and Sertoli cells | Female germ cells |
| Anti-BRCA1 | ||||
| K-18* | hBRCA1 (aa 70-89) | Santa Cruz Biotechnologies | Cyt. (+) | Cyt. (+) |
| 8F7† | Fusion protein GST-hBRCA1 | GeneTex | N (+) | Cyt. (+), N (+) |
| (aa 341-748) | ||||
| 17F8† | Fusion protein GST-hBRCA1 | GeneTex | N (+) | Cyt. (+) |
| (aa 762-1315) | ||||
| 66036N* | hBRCA1 (aa 2-20) | Pharmingen | Cyt. (+), N (+) | Cyt. (+) |
| 66046N* | hBRCA1 (aa 768-793) | Pharmingen | Cyt. (+) | Cyt. (+) |
| 66056N* | hBRCA1 (aa 1847-1863) | Pharmingen | Cyt. (+) | Cyt. (++) |
| Anti-BRCA2 | ||||
| 66066E* | hBRCA2 (aa 1323-1346) | Pharmingen | Cyt. (+) | Cyt. (+) |
| 66076E* | hBRCA2 (aa 2586-2600) | Pharmingen | Cyt. (+) | Cyt. (+) |
| 5F6 (clone 1H3)† | Fusion protein GST-hBRCA2 | GeneTex | Cyt. (+) | Cyt. (+), N (+) |
| (aa 2336-2478) | ||||
| 3E6† | Fusion protein GST-hBRCA2 | GeneTex | Cyt. (+) | Cyt. (+) |
| (aa 188-563) | ||||
*Rabbit polyclonal antibodies and †mouse monoclonal antibodies. aa, Amino acid; Cyt., cytoplasm; N, nuclear; (+) intensive; (++), very intensive.